Genome annotation identified the presence of several viral open reading frames (ORFs) participating in metabolism, including a Sir2/cobB (sirtuin) protein and a number of genes involved in auxiliary NAD + and nucleotide biosynthesis, necessary for phage DNA replication. The function of many viral protein products in the process of infection is still unknown. Although many reports of newly sequenced viruses have provided a large set of information, basic research related to the shift of the bacterial metabolism during infection remains stagnant. Phylogenetic analysis revealed that they belong to the “schizoT4like” clade. Comparative genomic analysis with other large Vibrio phages was also performed to establish the presence and location of homing endonucleases highlighting distinct features for both phages. In addition, phage–host metabolic interactions and exploitation was studied by transcript profiling of selected viral and host genes. Sequencing and annotation was performed for two large double stranded DNA bacteriophages, φGrn1 and φSt2 of the Myoviridae family, considered to be of great interest for phage therapy against Vibrios in aquaculture live feeds. 3Marine Biological Section, University of Copenhagen, Helsingør, Denmark.2Institute of Marine Biology, Biotechnology and Aquaculture, Hellenic Centre for Marine Research, Heraklion, Crete, Greece.1Laboratory of Molecular Biology, Department of Biotechnology, School of Food, Biotechnology and Development, Agricultural University of Athens, Athens, Greece.Kalatzis 2,3 Pantelis Katharios 2 Emmanouil Flemetakis 1*
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